Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).
نویسندگان
چکیده
An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase variant with improved catalytic activity.
منابع مشابه
Fluorescence-activated cell sorting and directed evolution of a-N-acetylgalactosaminidases using a quenched activity-based probe (qABP)†
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ورودعنوان ژورنال:
- Chemical communications
دوره 49 65 شماره
صفحات -
تاریخ انتشار 2013